https://hdl.handle.net/2238/3288 Artículos en otras revistas 2026-05-21T05:39:38Z https://hdl.handle.net/2238/16432 Physicochemical and microbial properties of dairy barn soils: a case study in Costa Rican farm-associated soils harboring the foodborne pathogen Listeria monocytogenes Guillén-Watson, Rossy; Barboza-Fallas, Luis; Masís-Meléndez, Federico; Rivas-Solano, Olga; Aguilar-Rodríguez, Rodrigo; Medaglia-Mata, Alejandro; Abanto, Michel; Núñez-Montero, Kattia Animal farming activities can influence soil properties that contribute to the survival of foodborne pathogens like the ubiquitous Listeria monocytogenes. However, the presence of this pathogen in farm-associated soils in Costa Rica has not been studied, which might provide new insights regarding the environmental conditions associated with the establishment of this pathogen. In this work, we correlated the presence of L. monocytogenes with the soil physicochemical properties and bacterial community structure of soils associated with livestock activities, including a dairy barn floor (DB) and a slaughterhouse holding pen (SH). A cropland (CL) was included as an example of soil not associated with animal farming practices. We characterized the presence pattern of L. monocytogenes via culture-dependent and culture-independent techniques (i.e., metabarcoding based on 16S rRNA gene sequencing) and conducted a determination of physical, elemental and chemical parameters with Fourier-transform infrared spectroscopy (FT-IR) to statistically determine the soil properties that correlate with L. monocytogenes’ presence in the soil. L. monocytogenes was isolated from DB samples and SH but not from CL. Subsequently, 16S rRNA gene-based metabarcoding showed that the presence of L. monocytogenes was positively correlated with higher bacterial diversity, while physicochemical analyses revealed that the total hydrogen and nitrogen contents of soil organic matter, pH, and electrical conductivity were the main drivers of L. monocytogenes’ presence. Moreover, a CL sample fertilized with animal-derived products showed DB-like physicochemical properties matching conditions in favor of L. monocytogenes’ presence. Hence, our work emphasizes the significance of soil as a primary source for the widespread dissemination of pathogens, particularly underscoring the necessity for improved agricultural practices to prevent cross-contamination with L. monocytogenes. Additionally, we highlight the importance of further understanding the biotic and abiotic factors in facilitating the establishment of L. monocytogenes. Artículo. 2023-01-01T00:00:00Z https://hdl.handle.net/2238/16324 Muscle-like scaffolds for biomechanical stimulation in a custom-built bioreactor Rojas-Rojas, Laura; Espinoza-Álvarez, María Laura; Castro-Piedra, Silvia; Ulloa-Fernández, Andrea; Vargas-Segura, Walter; Guillén-Girón, Teodolito Tissue engineering aims to develop in-vitro substitutes of native tissues. One approach of tissue engineering relies on using bioreactors combined with biomimetic scaffolds to produce study models or in-vitro substitutes. Bioreactors provide control over environmental parameters, place and hold a scaffold under desired characteristics, and apply mechanical stimulation to scaffolds. Polymers are often used for fabricating tissue-engineering scaffolds. In this study, polycaprolactone (PCL) collagen-coated microfilament scaffolds were cell-seeded with C2C12 myoblasts; then, these were grown inside a custom-built bioreactor. Cell attachment and proliferation on the scaffolds were investigated. A loading pattern was used for mechanical stimulation of the cell-seeded scaffolds. Results showed that the microfilaments provided a suitable scaffold for myoblast anchorage and that the custom-built bioreactor provided a qualified environment for the survival of the myoblasts on the polymeric scaffold. This PCL-based microfilament scaffold located inside the bioreactor proved to be a promising structure for the study of skeletal muscle models and can be used for mechanical stimulation studies in tissue engineering applications. Artículo. Instituto Tecnológico de Costa Rica (ITCR). Escuela de Ciencia e Ingeniería de los Materiales, Escuela de Física, Escuela de Biología, 2022 2022-01-01T00:00:00Z https://hdl.handle.net/2238/14915 Competencias de comportamiento, determinando la existencia de una brecha entre la academia y el mercado laboral de TI Ortiz-Acuña, Cindy; Solano-Cordero, Jennier; Ferreto-Gutiérrez, Eugenia; Rojas-Segura, Javier Este estudio tiene como objetivo identificar las competencias de comportamiento relevantes para los profesionales de las tecnologías de la información (TI) y determinar si existe una brecha entre las competencias desarrolladas por la academia y las requeridas por el mercado laboral en Costa Rica. Para ello, se llevó a cabo un análisis que involucró a cuatro grupos de interés: la literatura, las bolsas de empleo, los perfiles de egreso de las carreras de TI en universidades públicas, así como a los directores y coordinadores académicos de dichas universidades. Durante este análisis, se identificaron 45 competencias de comportamiento, de las cuales solo tres coincidieron en su categorización como de alto o moderado grado de importancia. Cabe destacar que no se encontró un conjunto homogéneo de competencias para todos los grupos investigados.; In order to identify the relevant behavioral competencies for IT professionals and determine if there is a gap between the competencies developed by academia and those required by the job market in Costa Rica, this article analyses four stakeholder groups: literature, job boards, the graduation profiles of IT programs in public universities, and the directors and academic coordinators of these universities. The analysis identified 45 behavioral competencies, of which only three coincided in their categorization as having a high or moderate degree of importance. Notably, the analysis found no homogeneous competencies for all the investigated groups. Artículo. Instituto Tecnológico de Costa Rica. Vicerrectoría de Investigación y Extensión (VIE). Dirección de proyectos. Escuela de Administración de Empresas, Escuela de Ingeniería en Computación, Centro de Investigación en Administración, Economía y Gestión Tecnológica, 2023 2023-01-01T00:00:00Z https://hdl.handle.net/2238/13324 La matemática como dominio masculino: un estudio de la percepción en la educación media costarricense Meza-Cascante, Luis Gerardo; Suárez-Valdés-Ayala, Zuleyka; Agüero-Calvo, Evelyn; Jiménez-Céspedes, Rodolfo; Calderón-Ferrey, Martha; Sancho-Martínez, Laura; Pérez-Tyteca, Patricia; Monje-Parrilla, Javier Se presentan los resultados de un estudio de la percepción de la matemática como dominio masculino, en estudiantes de educación media oficial diurna costarricense, realizado con la intención de evaluar la necesidad de desarrollar programas de intervención que promuevan las mismas competencias en las mujeres que los hombres en matemática. Se entiende como “dominio masculino” la creencia de que los hombres presentan mejores condiciones que las mujeres para aprender matemática y, en general, para desempeñarse en ambientes relacionados con esa disciplina.; The results of a study on the perception of mathematics as a male domain in official day high school students in Costa Rica are presented. The study was conducted to evaluate the need to develop intervention programs that promote that women have the same capabilities as men in mathematics. “Male domain” is understood as the belief that men have better conditions than women to learn mathematics and, in general, to perform in environments related to that discipline. Revista Electrónica Educare (Educare Electronic Journal) EISSN: 1409-4258 Vol. 25(3) SETIEMBRE-DICIEMBRE, 2021: 1-15 2021-01-01T00:00:00Z