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dc.contributor.authorSadeghi, Sara
dc.contributor.authorGarcía‑Molina, Almudena
dc.contributor.authorCelma, Ferran
dc.contributor.authorFereidounfar, Sogol
dc.contributor.authorSoler, Carles
dc.contributor.authorValverde-Abarca, Anthony
dc.date.accessioned2018-08-27T20:21:34Z
dc.date.available2018-08-27T20:21:34Z
dc.date.issued2016
dc.identifierhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5109872/es
dc.identifier.citationSadeghi, S., García-Molina, A., Celma, F., Valverde, A., Fereidounfar, S., & Soler, C. (2016). Morphometric comparison by the ISAS® CASA-DNAf system of two techniques for the evaluation of DNA fragmentation in human spermatozoa. Asian Journal of Andrology, 18(6):835-839.es
dc.identifier.urihttps://hdl.handle.net/2238/9959
dc.descriptionArtículo científicoes
dc.description.abstractDNA fragmentation has been shown to be one of the causes of male infertility, particularly related to repeated abortions, and different methods have been developed to analyze it. In the present study, two commercial kits based on the SCD technique (Halosperm ® and SDFA) were evaluated by the use of the DNA fragmentation module of the ISAS ® v1 CASA system. Seven semen samples from volunteers were analyzed. To compare the results between techniques, the Kruskal-Wallis test was used. Data were used for calculation of Principal Components (two PCs were obtained), and subsequent subpopulations were identified using the Halo, Halo/Core Ratio, and PC data. Results from both kits were significantly different (P < 0.001). In each case, four subpopulations were obtained, independently of the classification method used. The distribution of subpopulations differed depending on the kit used. From the PC data, a discriminant analysis matrix was obtained and a good a posteriori classification was obtained (97.1% for Halosperm and 96.6% for SDFA). The present results are the first approach on morphometric evaluation of DNA fragmentation from the SCD technique. This approach could be used for the future definition of a classification matrix surpassing the current subjective evaluation of this important sperm factor.es
dc.language.isospaes
dc.publisherInstituto Tecnológico de Costa Rica. Escuela de Agronomía. Sede San Carlos.es
dc.relation.hasversion10.4103/1008-682X.186875es
dc.sourceAsian Journal of Andrologyes
dc.subjectÁcido desoxirribonucleicoes
dc.subjectSeres humanoses
dc.subjectFertilidades
dc.subjectMetabolismoes
dc.subjectResearch Subject Categories::FORESTRY, AGRICULTURAL SCIENCES and LANDSCAPE PLANNING::Plant production::Agronomyes
dc.titleMorphometric comparison by the ISAS® CASA-DNAf system of two techniques for the evaluation of DNA fragmentation in human spermatozoaes
dc.typeartículo originales


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