Effect of growth conditions on Ophiostoma piceae (Münch) H. & P. Syd. and Ophiostoma floccosum Math-Käärik albino strains culture morphology

Berrocal-Jiménez, Alexander; Navarrete, José; Oviedo, Claudia

dc.creator	Berrocal-Jiménez, Alexander
dc.creator	Navarrete, José
dc.creator	Oviedo, Claudia
dc.date	2014-12-29
dc.date.accessioned	2021-06-08T22:00:57Z
dc.date.available	2021-06-08T22:00:57Z
dc.identifier	https://revistas.tec.ac.cr/index.php/kuru/article/view/2095
dc.identifier	10.18845/rfmk.v12i28.2095
dc.identifier.uri	http://hdl.handle.net/2238/12605
dc.description	Blue stain in Pinus radiata D. Don wood is predominantly caused by Ophiostoma genus fungi. Stained wood causes significant economic losses to forestry and timber industries. Currently, different chemical fungicides are used to prevent the occurrence of blue stain fungi. However, recent reports have questioned the environmental friendliness of these fungicides. For this reason, the use of biological control, in particular with albino strains of the Ophiostoma genus, appears to be an environmentally safe and a technically feasible alternative to work with. The potential field application of this technology would use a formulation containing the albino fungus-the bioactive ingredient- in its yeast like form. However, some Ophiostoma strains cultures present a marked filamentous morphology in liquid culture medium, affecting the efficiency in the production of the bioactive ingredient. The hypothesis of this work presumed the possibility to control the morphology of Ophiostoma cultures, through the modification of growth conditions in liquid medium. The effect of inoculum size, growth temperature and agitation rate on the morphology of PcF2A29 Ophiostoma piceae and FlF1A55 Ophiostoma floccosum albino strains in liquid culture medium, was studied. A 23 factorial design was employed. The results showed that the initial inoculum size had a statistically significant effect on yeast-like growth morphology in both strains, whereas the temperature only produced a significant effect in PcF2A29 O. piceae strain.	en-US
dc.description	Blue stain in Pinus radiata D. Don wood is predominantly caused by Ophiostoma genus fungi. Stained wood causes significant economic losses to forestry and timber industries. Currently, different chemical fungicides are used to prevent the occurrence of blue stain fungi. However, recent reports have questioned the environmental friendliness of these fungicides. For this reason, the use of biological control, in particular with albino strains of the Ophiostoma genus, appears to be an environmentally safe and a technically feasible alternative to work with. The potential field application of this technology would use a formulation containing the albino fungus-the bioactive ingredient- in its yeast like form. However, some Ophiostoma strains cultures present a marked filamentous morphology in liquid culture medium, affecting the efficiency in the production of the bioactive ingredient. The hypothesis of this work presumed the possibility to control the morphology of Ophiostoma cultures, through the modification of growth conditions in liquid medium. The effect of inoculum size, growth temperature and agitation rate on the morphology of PcF2A29 Ophiostoma piceae and FlF1A55 Ophiostoma floccosum albino strains in liquid culture medium, was studied. A 23 factorial design was employed. The results showed that the initial inoculum size had a statistically significant effect on yeast-like growth morphology in both strains, whereas the temperature only produced a significant effect in PcF2A29 O. piceae strain.	es-ES
dc.format	application/pdf
dc.language	eng
dc.publisher	Editorial Tecnológica de Costa Rica	es-ES
dc.relation	https://revistas.tec.ac.cr/index.php/kuru/article/view/2095/1931
dc.rights	acceso abierto	es-ES
dc.source	Revista Forestal Mesoamericana Kurú; Vol. 12 Núm. 28 (2015): Enero- Junio 2015; 4-12	es-ES
dc.source	2215-2504
dc.subject	Ophiostoma piceae	es-ES
dc.subject	Ophiostoma floccosum	es-ES
dc.subject	wood-staining fungi	es-ES
dc.subject	fungal dimorphism	es-ES
dc.subject	inoculum size	es-ES
dc.subject	Chile.	es-ES
dc.subject	Ophiostoma piceae	en-US
dc.subject	Ophiostoma floccosum	en-US
dc.subject	wood-staining fungi	en-US
dc.subject	fungal dimorphism	en-US
dc.subject	inoculum size	en-US
dc.subject	Chile.	en-US
dc.title	Effect of growth conditions on Ophiostoma piceae (Münch) H. & P. Syd. and Ophiostoma floccosum Math-Käärik albino strains culture morphology	en-US
dc.title	Effect of growth conditions on Ophiostoma piceae (Münch) H. & P. Syd. and Ophiostoma floccosum Math-Käärik albino strains culture morphology	es-ES
dc.type	artículo original

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