Establecimiento in vitro de Terminalia amazonia (Gmel.) Excell

Abstract

Terminalia amazonia is a native species used for reforestation because of their rapid growth characteristics and wood quality. The main objective of this study was to determine the conditions for the in vitro establishment of that species, the work was with plants from field and from greenhouse, to the last ones sanitary conditions were established prior disinfection. Two disinfectants agents were tested: sodium hypochlorite (NaClO) and mercuric chloride at different concentrations and exposure times. As the dual disinfection of 3% NaClO for 5 minutes and 1,5% for 10 minutes, was the best treatment to reduce contamination of the explants and keep them viable. Also a number of antioxidants was used, such as citric acid, ascorbic acid, L-cysteine, PVPP, activated charcoal and DTT, in addition was used a cold treatment at 4°C in order to inhibit phenolization of the explants. In the culture media, the addition of active charcoal and PVPP were the best treatments evaluated, with the lowest oxidation and the highest survival of explants (53,3% and 66,7% respectively). In addition, growth regulators as BAP and KIN were used to promote sprouting in the explants. The conditions for the establishment in vitro dependent directly on the species with which they work.