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Effect of growth conditions on Ophiostoma piceae (Münch) H. & P. Syd. and Ophiostoma floccosum Math-Käärik albino strains culture morphology

dc.creatorBerrocal-Jiménez, Alexander
dc.creatorNavarrete, José
dc.creatorOviedo, Claudia
dc.date2014-12-29
dc.date.accessioned2015-03-23T22:04:35Z
dc.date.available2015-03-23T22:04:35Z
dc.identifierhttp://revistas.tec.ac.cr/index.php/kuru/article/view/2095
dc.identifier.urihttp://hdl.handle.net/2238/5455
dc.descriptionBlue stain in Pinus radiata D. Don wood is predominantly caused by Ophiostoma genus fungi. Stained wood causes significant economic losses to forestry and timber industries. Currently, different chemical fungicides are used to prevent the occurrence of blue stain fungi. However, recent reports have questioned the environmental friendliness of these fungicides. For this reason, the use of biological control, in particular with albino strains of the Ophiostoma genus, appears to be an environmentally safe and a technically feasible alternative to work with. The potential field application of this technology would use a formulation containing the albino fungus-the bioactive ingredient- in its yeast like form. However, some Ophiostoma strains cultures present a marked filamentous morphology in liquid culture medium, affecting the efficiency in the production of the bioactive ingredient. The hypothesis of this work presumed the possibility to control the morphology of Ophiostoma cultures, through the modification of growth conditions in liquid medium. The effect of inoculum size, growth temperature and agitation rate on the morphology of PcF2A29 Ophiostoma piceae and FlF1A55 Ophiostoma floccosum albino strains in liquid culture medium, was studied. A 23 factorial design was employed. The results showed that the initial inoculum size had a statistically significant effect on yeast-like growth morphology in both strains, whereas the temperature only produced a significant effect in PcF2A29 O. piceae strain.
dc.descriptionBlue stain in Pinus radiata D. Don wood is predominantly caused by Ophiostoma genus fungi. Stained wood causes significant economic losses to forestry and timber industries. Currently, different chemical fungicides are used to prevent the occurrence of blue stain fungi. However, recent reports have questioned the environmental friendliness of these fungicides. For this reason, the use of biological control, in particular with albino strains of the Ophiostoma genus, appears to be an environmentally safe and a technically feasible alternative to work with. The potential field application of this technology would use a formulation containing the albino fungus-the bioactive ingredient- in its yeast like form. However, some Ophiostoma strains cultures present a marked filamentous morphology in liquid culture medium, affecting the efficiency in the production of the bioactive ingredient. The hypothesis of this work presumed the possibility to control the morphology of Ophiostoma cultures, through the modification of growth conditions in liquid medium. The effect of inoculum size, growth temperature and agitation rate on the morphology of PcF2A29 Ophiostoma piceae and FlF1A55 Ophiostoma floccosum albino strains in liquid culture medium, was studied. A 23 factorial design was employed. The results showed that the initial inoculum size had a statistically significant effect on yeast-like growth morphology in both strains, whereas the temperature only produced a significant effect in PcF2A29 O. piceae strain.
dc.formatapplication/pdf
dc.languageeng
dc.publisherEditorial Tecnológica de Costa Rica
dc.relationhttp://tecdigital.tec.ac.cr/servicios/ojs/index.php/kuru/article/view/2095/1931
dc.rightsCopyright (c) 2014 Revista Forestal Mesoamericana Kurú
dc.sourceRevista Forestal Mesoamericana Kurú; Vol. 12, Núm. 28 (2015); 4-12
dc.source2215-2504
dc.subjectOphiostoma piceae, Ophiostoma floccosum, wood-staining fungi, fungal dimorphism, inoculum size, Chile.
dc.subjectOphiostoma piceae, Ophiostoma floccosum, wood-staining fungi, fungal dimorphism, inoculum size, Chile.
dc.titleEffect of growth conditions on Ophiostoma piceae (Münch) H. & P. Syd. and Ophiostoma floccosum Math-Käärik albino strains culture morphology
dc.titleEffect of growth conditions on Ophiostoma piceae (Münch) H. & P. Syd. and Ophiostoma floccosum Math-Käärik albino strains culture morphology
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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