Identificación de factores de transcripción putativos en Stevia rebaudiana y Tagetes ratula como herramienta para posterior uso en la descripción de rutas metabólicos de interés
Torres-Rojas, Francisco José
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In recent years, there has been increasing interest in exploiting and maximize use of medicinal plants and / or secondary metabolites, however, this has triggered turn overexploitation of certain species, threatening their survival. Currently the basic and applied research have combined to achieve propagate species with medicinal potential and to elucidate the relevant biosynthetic pathways, with a view to its potentiation. This research aimed to identify gene sequences related to putative transcription factors and Tagetes patula Stevia rebaudiana. In the case of S. rebaudiana, 15genes metabolic synthesis pathway glycosides were present in the RNA sample sequenced; T. patula was obtained than those reported for this species in the NCBI, genes having a transcription factor also were present in the biological material worked. For Tagetes patula, favorable for the identification of a transcription factor which is not defined function satisfactorily in databases result was obtained. As alignments against transcription factors described species of the Asteraceae family, the results were negative for identifying any of them within the transcriptome of T. patula and S. rebaudiana. Future gene expression analysis by cDNA sequencing would be more accurate by assembling a transcriptome de novo from a complete sequencing.
Proyecto de Investigación VIE-5401-1510-9301