Optimization CASA-Mot analysis of donkey sperm: optimum frame rate and values of kinematic variables for different counting chamber and fields

Abstract

A reliable sperm motility exam is important for semen analysis and breeding soundness examination. Different parameters can affect the Computer Assisted Sperm Analysis (CASA) motility results. Today, new high-resolution cameras and different chambers are introduced to CASA systems, and protocol optimization is required to render the estimation results for donkey sperm. The objective of this study is the optimization of the conditions used for donkey semen motility analysis with CASA-Mot by defining the optimum frame rate for different chamber types. Additionally, to study the effect of different chamber types, chamber field and sperm dilution on the sperm kinematic parameters with higher frame rates are examined.